Functionalized large pore mesoporous silica\ud nanoparticles for gene delivery featuring controlled\ud release and co-delivery

摘要

Novel mesoporous silica nanoparticles (LPMSNs) functionalised with degradable poly(2-dimethylaminoethyl\udacrylate) (PDMAEA) have been developed (PDMAEA–LPMSNs) as nano-carriers for gene\uddelivery. The unique design of PDMAEA–LPMSNs has endowed this system with multiple functions\udderived from both the organic and inorganic moieties. The cationic polymer unit binds to genetic\udmolecules and undergoes a self-catalyzed hydrolysis in water to form a non-toxic anionic polymer\udpoly(acrylic acid), allowing controlled release of siRNA in the cells. The nanopores of the LPMSNs provide\uda reservoir for storage and release of chloroquine to facilitate endosomal escape. The PDMAEA–LPMSN\udcomposites were characterized by elemental analysis (EA), X-ray photoelectron spectroscopy (XPS),\udsolid-state 13C magic-angle spinning nuclear magnetic resonance (MAS-NMR), thermogravimetric\udanalysis (TGA), and nitrogen sorption techniques. Their siRNA delivery performance was tested in a KHOS\udcell line, showing promising potential for co-delivery of genes and drugs.